It may be required, I would have to confirm. It is usually part of my routine to perform the first homing action via the touchscreen when the printer is powered on. However I don’t know the exact GCode it emits.
I would also suggest you follow the LightBurn guide from @Skreelink (Full Lightburn Control Guide) first. The “Start GCode” of your device settings should contain GCode that sets the Z-axis correctly.